Veterinary

Findings from Chonbuk National University Broaden Understanding of Salmonella enteritidis

  2012 JAN 30 - (VerticalNews.com) -- "A novel Salmonella Enteritidis (SE) vaccine candidate strain, JOL919 was constructed by deleting the Ion and cpxR genes from a wild-type SE using an allelic exchange method. The study was carried out to evaluate the strain as a vaccine candidate against salmonellosis," scientists writing in the journal Veterinary Immunology and Immunopathology report.

  "The strain showed the enhanced macrophage invasion, early bacterial clearance and higher immune responses as compared to the other mutants, JOL917 (Delta lon) and JOL918 (Delta cpxR), and the wild type. In further analysis, the chickens immunized with JOL919 showed a significant increase in plasma IgG and intestinal secretory IgA levels, which was an indication of robust humoral and mucosal immune responses induced by the candidate. The lymphocyte proliferation response and CD45(+)CD3(+) T cells, associated with an activation of T helper and cytotoxic cells, were also significantly increased in the immunized group, which indicated that the candidate also induced cellular immune responses. The immune cell influx into caecal tissues analyzed by immunohistochemistry showed that CD8(+) T cells were predominated in the immunized group, suggesting that the candidate can clear the invaded pathogen in the intestines by a more direct way involving cytotoxic activity," wrote R.M. Nandre and colleagues, Chonbuk National University.

  The researchers concluded: "By the examination of the protection efficacy measured by observations of gross lesions in the organs and bacterial recovery, the candidate can provide an efficient protection upon virulent challenge."

  Nandre and colleagues published their study in Veterinary Immunology and Immunopathology (Immunogenicity of a Salmonella Enteritidis mutant as vaccine candidate and its protective efficacy against salmonellosis in chickens. Veterinary Immunology and Immunopathology, 2011;144(3-4):299-311).

  Additional information can be obtained by contacting R.M. Nandre, Chonbuk National University, Coll Vet Med, Jeonju 561756, South Korea.

  The publisher of the journal Veterinary Immunology and Immunopathology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands.

  Keywords: City:Jeonju, Country:South Korea, Region:Asia, Veterinarian, Enterobacteriaceae, Gammaproteobacteria, Salmonella enterica, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods

  This article was prepared by VerticalNews Veterinary editors from staff and other reports. Copyright 2012, VerticalNews Veterinary via VerticalNews.com.